Coding

Part:BBa_K1808009

Designed by: Remsha Afzal   Group: iGEM15_TrinityCollegeDublin   (2015-09-16)


CYTOCHROME P450 REDUCTASE HIS TAGGED

This is the coding region of the CPR gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone as well as a 6 his tagged component. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CPR gene encodes the enzyme Cytochrome P450 reductase, which catalyses the conversion of NADPH back to NAD+ by transference of electron between Cytochrome P450. The product is used in the production of dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 962
    Illegal AgeI site found at 1687
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1834


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Categories
Parameters
None